Discovery Kit™: dSTORM in Cells (Strong Fixative)

Kits

$648.00

The kit to try for membrane & structural proteins

  • Label targets of interest with your own primary antibodies
  • Use highly specific anti-mouse, rabbit and rat secondary antibodies conjugated with best-in-class dSTORM fluorophores
  • Optimize key sample preparation steps, such as fixation, to preserve biological structures and avoid artifacts
  • Perform 2-color super-resolution dSTORM imaging in various cell types
  • Obtain images with 20 nm resolution using ONI’s Nanoimager
  • Use ONI’s CODI platform for cluster-based data analysis to extract novel nanoscale cellular information
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Minimum Purchase:
1 unit
Maximum Purchase:
4 units
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About

Sample preparation for single molecule imaging is more challenging than for confocal microscopy. The ONI Discovery Kit: dSTORM in Cells includes all the reagents needed to label your proteins of interest and image them using dSTORM, a single-molecule localization microscopy technique. All these reagents have been tested and optimized so that newcomers to super-res can acquire top quality dSTORM images.

PFA can be insufficient to cross-link many membrane proteins, which remain mobile. This can lead to antibody-induced protein clustering. The dSTORM Discovery Kit with strong fixative immobilizes membrane proteins using a combination of PFA and glutaraldehyde. This better preserves the native structure and enables you to confidently measure membrane protein clustering. Because glutaraldehyde can disrupt some epitopes this kit provides both fixatives for you to compare and choose the best fixative for your protein of interest.

Microscope systems with equivalent specifications and compatible with dSTORM imaging can be used to image samples prepared using the dSTORM Discovery Kit.

In the box

  • 50x 100 µL labeling reactions
  • PFA-based fixative and fixation supplement for strong fixation
  • Wash buffer
  • Blocking buffer
  • Staining buffer
  • Quenching buffer
  • Permeabilization Concentrate
  • Choice of two secondary F(ab’)2 antibodies (mouse, rabbit or rat) with either CF583R or AZ647 (same molecule as Alexa FluorTM 647)BCubed imaging buffer
  • Comprehensive protocol

Specifications

  • Strorage: Store at -20 ºC
  • Shelf life: BCubed buffer has a shelf-life of two months and the rest of the kit has a shelf-life of 6 months
  • Shipping: Item ship in two separate boxes based on temperature storage needs
  • Processing time: Please allow a processing time of 5-10 business days before your items are shipped

Please visit product webpage for more information: Discovery Kit: dSTORM

FAQs

How many samples can I stain with each kit?
The kit contains sufficient reagents for 50 100 µl samples (for example Ibidi 18 well slide)

What’s the kit’s shelf life?
BCubed buffer has a shelf-life of 2 months and the rest of the kit has a shelf-life of 6 months. BCubed can be independently ordered from the rest of the kit in case it expires before you have finished the kit.

Resources

References

  • Nerreter T, Letschert S, Götz R, Doose S, Danhof S, Einsele H, Sauer M, Hudecek M. Super-resolution microscopy reveals ultra-low CD19 expression on myeloma cells that triggers elimination by CD19 CAR-T. Nat Commun. 2019; 10(1): 3137. doi: 10.1038/s41467-019-10948-w. 
  • Tanaka, K., Suzuki, K., Shirai, Y. et al. Membrane molecules mobile even after chemical fixation. Nat Methods. 2010; 7, 865–866. https://doi.org/10.1038/nmeth.f.314
  • Stanly TA, Fritzsche M, Banerji S, García E, Bernardino de la Serna J, Jackson DG, Eggeling C. Critical importance of appropriate fixation conditions for faithful imaging of receptor microclusters. Biol Open. 2016; 5(9): 1343-50. doi: 10.1242/bio.019943.
  • Werner C, Sauer M and Geis C. Super-resolving Microscopy in Neuroscience. Chem. Rev. 2021; 121 (19): 11971–12015.