Application Kit™: EV Profiler 2 - Tetraspanin Profiling



Label, image and analyze EVs in super-resolution

  • Visualize EVs and their associated biomarkers in super-resolution
  • Co-localize tetraspanins CD9, CD63 and CD81 with ONI-validated antibodies
  • Quantify EV phenotype and size at a single EV and population level
  • Choose between ONI-validated capture and detection antibodies or use your antibodies of choice
  • User-friendly imaging setup, accessible to all levels of expertise
  • Reduced hands-on time with automated acquisition in AutoEV
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Minimum Purchase:
1 unit
Maximum Purchase:
6 units
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EV Profiler 2: Tetraspanin Profiling is a reagent kit for visualization and phenotyping of human tetraspanins CD9, CD63 and CD81 on extracellular vesicles using dSTORM microscopy on the Nanoimager.

Visualize and quantify tetraspanins or your biomarker of interest on individual EVs in stunning 3 color super resolution microscopy.

Capture up to 3x more EVs per chip compared to EV Profiler 1 and measure biomarker positivity with a 20% CV. When combined with our newest software offering, AutoEV*, your system will be calibrated and optimized to acquire and analyze a 4 lane chip automatically and deliver a comprehensive report of EV size and positivity for each individual lane in less than one hour.

Using with Aplo Flow? Don't forget the Automation Accessory Kit available here

*AutoEV requires a CODI account and an active internet connection. Access to AutoEV without an internet connection will come later in 2024. AutoEV is only compatible with Nanoimagers manufactured from 2021 on.

In the box

Box 1

  • A choice of different proteins for capture (3 tubes)
    (CD9, CD63, CD81, Tetraspanin Trio) 
  • 3 Color Tetraspanin Antibody Panel (CD9, CD63 and CD81)
  • Fixative, Wash Buffer and Staining Buffer
  • Permeabilization Buffer
  • EV Standard Control (lyophilised EVs from HCT-116 culture media) (add on)

Box 2

  • dSTORM Imaging Buffer
  • Surface Reagent
  • Phosphatidylserine capture (if selected)

Assay Chips

  • 3 Assay Chips, each with four lanes



  • Storage: Box 1: 4 ºC | Box 2 & Assay Chips: -20 ºC
  • Shelf life: 2 months
  • Shipping: Box 1 & Assay Chips on Wet Ice | Box 2 on Dry Ice
  • Processing time: Please allow a processing time of 5-10 business days before your items are shipped

Please visit product webpage for more information: EV Profiler 2 with AutoEV


What are the software requirements for the use of AutoEV?
AutoEV requires a CODI account and an active internet connection. Access to AutoEV without an internet connection will come later in 2024. AutoEV supports most Nanoimagers manufactured after 2021.

What type of EVs is the kit compatible with?
The kit was validated for human-derived EVs.

What EV purification methods should I use?
Size exclusion chromatography purified or differential centrifugation.

What’s the kit’s shelf life?
The shelf life of the kit is 2 months

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